Microscopy Core Facilities of the NCI

Microscopy
Core
Facilities
NIH:NCI:DBS

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For NIH wide microscopy contacts and activities please see the website of the
LIGHT MICROSCOPY INTEREST GROUP

Facility	Fluorescence Imaging Facility	CCR Confocal Microscopy Core Facility	Microscopy and Digital Imaging Facility	Confocal Microscopy Laboratory @ Frederick	Electron Microscopy Laboratory @ Frederick	LCMB Microscopy Core	CCBB Microinjection and Confocal Microscopy Core
Lab/Branch	Laboratory of Receptor Biology and Gene Expression	Laboratory of Experimental Carcinogenesis	Experimental Immunology Branch	NCI-FCRDC	NCI-FCRDC	Laboratory of Cellular and Molecular Biology	Cell and Cancer Biology Branch
Location (Bldg/Rm)	41/C-615	37/4137	10/4A05	538/157		37/2033B	37/1035
Contact	James McNally (301) 402-0209 mcnallyj@exchange.nih.gov	Susan Garfield (301) 496-5688 x227 susan_garfield@nih.gov	Michael Kruhlak (301) 435-6402 Michael_Kruhlak@nih.gov	Stephen Lockett (301) 846-5515 slockett@ncifcrf.gov	Kunio Nagashima 301-846-1594 nagashim@mail.ncifcrf.gov	Valarie Barr 301-496-9052 barrv@mail.nih.gov	Yvona Ward 301-594-2645 yward@mail.nih.gov
Confocal microscopes	Leica SP / Leica confocal software laser lines: UV 351 nm Argon 488 nM, 514 nm Krypton 568 nm HeNe 633 nM Zeiss LSM510-ConfoCor / Zeiss LSM510 confocal software laser lines: Argon 458 mn, 488 nm, 514 nm HeNe1 543 nm HeNe2 633 nm Zeiss LSM510-META/ Zeiss LSM510 confocal software laser lines: Laser module 405 nM Argon 458 mn, 488 nm, 514 nm HeNe1 543 nm HeNe2 633 nm Atto Bioscience CARV spinning disk confocal/ Metamorph software Mercury lamp DAPI, FITC, TexRed, Cy5, CFP, YFP filter set	Zeiss 510 NLO (2-photon) Meta (inverted)/Zeiss AIM software laser lines: Chameleon IR (2-photon) 720 nm to 930 nm Argon 458 nm, 488 nm, 514 nm HeNe1 543 nm HeNe2 633 nm Zeiss 510 UV (inverted)/Zeiss AIM software laser lines: UV: 364 nm Argon 458 nm, 488 nm, 514 nm HeNe1 543 nm HeNe2 633 nm Zeiss/Bio-Rad MRC 1024 (upright)/Bio-Rad LaserSharp software laser lines: Krypton/Argon 488 nm, 568 nm, 647 nm	Zeiss LSM 510 META (inverted) laser lines: UV (351 and 364nm) VIS Ar multi-line (458, 477, 488, and 514nm) VIS HeNe 543 and HeNe 633	IAL - NCI-Frederick Confocal Microscopes Located in Building 538-Rm 157 Zeiss 510 META NLO (Inverted) Laser Lines: Ar Multi-Line - 458, 477, 488, 514 HeNe - 543 HeNe - 633 Two-Photon - 780-1000 Zeiss 510 META (Inverted) Laser Lines: Diode - 405 Ar Multi-Line - 458, 477, 488, 514 DPSS - 561 HeNe - 594 Olympus FV1000 (Inverted) (On order) Laser Lines: Diode - 405 AOTF - 458/488/515/561/633 Other Microscopes Located in Building 560-Rm Zeiss 510 (Inverted) (On order) Laser Lines: Diode - 405 Ar Multi-Line - 458, 477, 488, 514 DPSS - 561 HeNe - 594 Zeiss 410 (Inverted) Laser Lines: UV 413 Green - 488 Red - 543/568 Far Red - 647	Hitachi H-7000 transmission electron microscope S-570 scanning electron microscopes Ultra microtomes and vibration-proof table for sectioning	Zeiss LSM510-META/ Zeiss LSM510 confocal software laser lines: Laser module 405 nm Argon 458 mn, 488 nm, 514 nm HeNe1 543 nm HeNe2 633 nm UltraView Spinning Disk / PerkinElmer confocal software laser lines: Cad 442 nm Argon 488 nM, 514 nm Argon 568 nm, 633 nm HeNe2 633 nm	Zeiss LSM 510 (inverted) Zeiss Aim software laser lines: UV 351, 364 nm Argon 458, 488, 514 nm HeNe 543 nm HeNe 633 nm
Deconvolution microscopes	Applied Precision Deltavision System		Zeiss Axioplan and Zeiss Axovert are equipped with motorized z-stage and can be used for basic deconvolution
Fluorescence microscopes	Leica DMRA upright microscope, Cool Snap ES camera/ Metamorph software Olympus IX81 inverted microscope, Pentamax 512 camera, DualView CFP-YFP / Metamorph software Nikon E3000 inverted microscope, CoolSnap ES camera, Dual View GFP-DsRed/ Metamorph software	Nikon (upright)	Zeiss Axioplan 2 (upright) with Hamamatsu ORCA ER and SPOT-2 cameras Zeiss Axiovert 200M (inverted) with CoolSnap ES camera and CARV spinning disk confocal module		Zeiss Axioskop light microscope	Olympus Vanox upright microscope, Cool Snap ES camera, IPLabs software	Zeiss Axiovert 100M (inverted) UV, Green and Red filters Eppendorf Microinjection System Nikon Digital Camera Zeiss Axioplan 2 (upright) Blue, Red, Green, and Triple pass filters Nikon DS Camera
Image Processing	Windows 2000/ Imaris software Windows 2000/ Metamorph software SGI O2 / Deconvolution (Delta Vision) software	Windows XP/Imaris v4.2 Windows XP/ImageProPlus v5.1, 3D Constructor v5.1, IQbase database software Windows XP/ImageAccess v4r123 database software	SGI NT 540 Analysis Workstation Software: Imaris (with Surpass and Colocalization modules) MetaMorph Slidebook	Pentium PC Macintosh SGI Indigo Unix Workstation	Digital image processing Darkroom photographic equipment	Windows XP/ Imaris software Windows XP/ Circsoft PFRET software Windows XP/ Full Zeiss processing module	Dual monitor workstation Adobe Illustrator Adobe Photoshop Zeiss processing

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